Cytochrome c modified by digestion with proteolytic enzymes: 2. Properties of pepsin-modified cytochrome c

Cytochrome c modified by digestion with proteolytic enzymes. 2. Properties of pepsin-modified cytochrome c.

Modified by Digestion with Proteolytic Enzymes

Although the importance of prosthetic groups to the activity of certain enzymes has been much studied, the part played by the protein portion of the molecule has received little attention. Haemoglobin, catalase and peroxidase all have the same prosthetic group, namely, protohaematin, yet the attachment of different proteins gives them distinct and characteristic properties. Thus haemoglobin is ...

The preparation and some properties of mammalian cytochrome c modified with 2-hydroxy-5-nitrobenzyl bromide.

2-Hydroxy-5-nitrobenzyl bromide reacts with horse heart cytochrome c at acid pH to yield a chemically modified protein. Chromatography of the protein on CM-cellulose allows separation of a single chemically modified species. This species is shown by gel chromatography to be monomeric, and isoelectric focusing shows the pI to be lowered from 10.5 to 9.8 on introduction of the reagent molecule. T...

Adsorption of cytochrome c by succinic anhydride-modified peanut shells

A novel adsorbent based on peanut shells modified with glutaraldehyde and succinic anhydride was prepared. Factors affecting the adsorption capacity, such as the pH, temperature, adsorption time, initial cytochrome c (cyt c) concentration and NaCl ionic strength, were extensively investigated. The results showed that the maximum adsorption capacity of the modified peanut shells (MPSs) was 432.6...

Some electron-transfer reactions involving carbodi-imide-modified cytochrome c.

The reaction kinetics of native and carbodi-imide-modified tuna and horse heart cytochromes c with both a strong (dithionite) and a relatively weak (ascorbate) reducing agent were studied over a wide range of conditions. In their reactions with dithionite both the native and modified cytochromes exhibit single exponential time courses. The effects of dithionite concentration and ionic strength ...